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Searching for F1 rollers and small worms On my final days in the Griffin lab, worms were injected with the SAS-6 injection mix that I had...

Last week, my supervisor and I decided to digest our SAS-4 plasmid to go from circular to linear. This may improve the efficiency of the...

Following my previous blog post, I had screened the F1 generation of the injected worms with SPD-2. I identified that 2 of the 100 worms...

After a few days of injecting the worms, and progeny was picked (by my supervisor), it was time to screen the progeny to see whether any...

Given the several failed amplifications of SAS-4 and SAS-6, my supervisor decided that it would be best to proceed with the injections...

As PCR of SPD-2 showed the cleanest band of all four genes, we proceeded with worm injections with SPD-2. After making the injection mix...

For the past 2-3 weeks, I have been altering PCR conditions to identify which conditions provide the cleanest and most desired DNA. This...

After the recieving plasmids from sequencing and confirming which plasmids actually contained the construct, I was able to select the...

After making cultures of bacteria with the desired plasmid and allowing the bacteria to grow, it was now time to extract the plasmids....

Previously, Golden Gate and adding homologous arms to our plasmids (containing HA-GFP tags to visualise translation live) was conducted....

Golden Gate Assembly For my project, golden gate assembly is required to introduce homology arms which are specific to the genes of...

One of the very first things I learnt in the lab was to pick and mount C. elegans for my future experiments. This technique is...