<?xml version="1.0" encoding="UTF-8"?><rss xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:content="http://purl.org/rss/1.0/modules/content/" xmlns:atom="http://www.w3.org/2005/Atom" version="2.0"><channel><title><![CDATA[AbbysURSSblog]]></title><description><![CDATA[Abbysurssblog]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/blog</link><generator>RSS for Node</generator><lastBuildDate>Mon, 19 Oct 2026 05:00:13 GMT</lastBuildDate><atom:link href="https://abelaineladinez.wixsite.com/abbysurssblog/blog-feed.xml" rel="self" type="application/rss+xml"/><item><title><![CDATA[swPCR of F1 SAS-6]]></title><description><![CDATA[Searching for F1 rollers and small worms On my final days in the Griffin lab, worms were injected with the SAS-6 injection mix that I had...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/swpcr-of-f1-sas-6</link><guid isPermaLink="false">68cd4be7d21422e7821829ea</guid><pubDate>Fri, 19 Sep 2025 12:33:48 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_ba05bb93019449beb277d06a6c8c9e63~mv2.png/v1/fit/w_1000,h_1000,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Digesting SAS-4 and Amplification]]></title><description><![CDATA[Last week, my supervisor and I decided to digest our SAS-4 plasmid to go from circular to linear. This may improve the efficiency of the...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/digesting-sas-4-and-amplification</link><guid isPermaLink="false">68cc31baaef6910f9dd38cab</guid><pubDate>Thu, 18 Sep 2025 16:41:28 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_030711d9abde46dda2b842840103fab2~mv2.png/v1/fit/w_1000,h_902,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[swPCR of SPD-2 F2 and F3]]></title><description><![CDATA[Following my previous blog post, I had screened the F1 generation of the injected worms with SPD-2. I identified that 2 of the 100 worms...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/swpcr-of-spd-2-f2-and-f3</link><guid isPermaLink="false">68cc296a4658226ab730908c</guid><pubDate>Thu, 18 Sep 2025 16:09:16 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_456e7f3aee32488a99ffb16e52b7005a~mv2.png/v1/fit/w_1000,h_789,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[swPCR of SPD-2 worms]]></title><description><![CDATA[After a few days of injecting the worms, and progeny was picked (by my supervisor), it was time to screen the progeny to see whether any...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/swpcr-of-spd-2-worms</link><guid isPermaLink="false">68c194f641d3328d56f65ff4</guid><pubDate>Thu, 11 Sep 2025 12:25:36 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_e4dbd46611c3400ca7b68a8b04757d39~mv2.png/v1/fit/w_1000,h_875,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Re-attempts of SAS-4 and SAS-6 amplification]]></title><description><![CDATA[Given the several failed amplifications of SAS-4 and SAS-6, my supervisor decided that it would be best to proceed with the injections...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/re-attempts-of-sas-4-and-sas-6-amplification</link><guid isPermaLink="false">68c1901329ab8110bd6440d5</guid><pubDate>Wed, 10 Sep 2025 15:08:29 GMT</pubDate><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Worm injections with SPD-2]]></title><description><![CDATA[As PCR of SPD-2 showed the cleanest band of all four genes, we proceeded with worm injections with SPD-2. After making the injection mix...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/worm-injections-with-spd-2</link><guid isPermaLink="false">68c17ad80effb1dd52560c35</guid><pubDate>Wed, 10 Sep 2025 14:48:21 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_28d4432798aa4aa5af3d9b3b2f7062f9~mv2.png/v1/fit/w_1000,h_1000,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Continuing Plasmid Amplification and Injection Mixes]]></title><description><![CDATA[For the past 2-3 weeks, I have been altering PCR conditions to identify which conditions provide the cleanest and most desired DNA. This...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/continuing-plasmid-amplification-and-injection-mixes</link><guid isPermaLink="false">68c045b279e788be281a9c84</guid><pubDate>Wed, 10 Sep 2025 13:18:17 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_a26a36ff27b644e7ab8fcd96734e45d6~mv2.png/v1/fit/w_1000,h_1000,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Plasmid Amplification with PCR]]></title><description><![CDATA[After the recieving plasmids from sequencing and confirming which plasmids actually contained the construct, I was able to select the...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/plasmid-amplification-with-pcr</link><guid isPermaLink="false">68a32a6734b07f0731c3ba99</guid><pubDate>Sat, 23 Aug 2025 16:02:31 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_c3f57581363a4313b7d798a0db120e55~mv2.png/v1/fit/w_600,h_304,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Plasmid Extraction using MiniPrep]]></title><description><![CDATA[After making cultures of bacteria with the desired plasmid and allowing the bacteria to grow, it was now time to extract the plasmids....]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/plasmid-extraction-using-miniprep</link><guid isPermaLink="false">6899ffd3fc810a67e31993f2</guid><pubDate>Mon, 11 Aug 2025 15:00:02 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_b988fb2f45d14b328325409ed5319409~mv2.png/v1/fit/w_1000,h_1000,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Tranfsorming Plasmids into E. coli and Culturing]]></title><description><![CDATA[Previously, Golden Gate and adding homologous arms to our plasmids (containing HA-GFP tags to visualise translation live) was conducted....]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/tranfsorming-plasmids-into-e-coli-and-culturing</link><guid isPermaLink="false">6899f90ebdacd23f8e8e162e</guid><pubDate>Mon, 11 Aug 2025 14:35:38 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_fa09f8c65e5d4ad3ad91c74f3b56197d~mv2.png/v1/fit/w_1000,h_1000,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Golden Gate Cloning]]></title><description><![CDATA[Golden Gate Assembly For my project, golden gate assembly is required to introduce homology arms which are specific to the genes of...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/golden-gate-cloning</link><guid isPermaLink="false">6899cdb1fc810a67e3190c02</guid><pubDate>Mon, 11 Aug 2025 14:04:49 GMT</pubDate><dc:creator>abelaineladinez</dc:creator></item><item><title><![CDATA[Picking and Mounting C. elegans]]></title><description><![CDATA[One of the very first things I learnt in the lab was to pick and mount C. elegans  for my future experiments. This technique is...]]></description><link>https://abelaineladinez.wixsite.com/abbysurssblog/post/picking-and-mounting-c-elegans</link><guid isPermaLink="false">6899cc9b4d23f3b3541a6301</guid><pubDate>Mon, 11 Aug 2025 10:57:33 GMT</pubDate><enclosure url="https://static.wixstatic.com/media/427119_01e63c87e5fa4525b1e1532d46199356~mv2.png/v1/fit/w_202,h_103,al_c,q_80/file.png" length="0" type="image/png"/><dc:creator>abelaineladinez</dc:creator></item></channel></rss>